Cryopreservation provides security against loss of valuable strains or lines of mice due to accident, disease, or disaster. It also provides a low-cost means of holding strains for future use that avoids the expense of per diem charges.
Cryopreservation of mouse strains involves the collection, freezing and storage of sperm or embryos in liquid nitrogen. A strain is considered to be truly cryopreserved when one is reasonably certain that one can recover mice from the frozen material; thus, a recovery step is done for quality control (QC) as part of the process. However, we will also freeze sperm without a this QC step at a considerably reduced cost. This provides a low-cost method of holding less valuable lines (for example, multiple lines carrying the same transgenic construct) for future use.
|Strain cryopreservation by sperm (with QC)||$1200|
|Strain cryopreservation by embryo (with QC)||$1200 + cost of mice and per diems|
|Sperm cryopreservation, no QC||$100/male|
|Recovery of live mice from cryopreserved sperm||$600 + cost of mice and per diems|
|Recovery of live mice from cryopreserved embryos||$400 + per diems|
|Shipment of frozen material||$100 + shipping charges|
Strain cryopreservation by sperm (with QC) Sperm from 3 or more males will be collected, pooled and cryopreserved. For quality contol, an aliquot will then be thawed and used to produce live mice.
Strain cryopreservation by embryo (with QC) Embyros will be produced by IVF or by natural matings and cryopreserved. For quality control, live mice from an aliquot of frozen embryos will be recovered. The investigator will be responsible for purchasing or supplying the mice used to produce the embryos, along with all associated per diem costs for their maintenance. At the request of the investigator, the recovered mice (these will be re-derived and thus pathogen-free) can be returned.
Sperm cryopreservation, no QC Sperm from male(s) will be collected, pooled and cryopreserved without attempting a recovery for quality control. Warning: There is no guarantee that live animals can be recovered from this frozen material. If the investigator decide at a future date to recover live animals from this frozen sperm, there will be a $400 surcharge to the usual recovery fee the first time this is done.
Recovery of live mice from cryopreserved sperm An aliquot of sperm will be thawed and used to recover live mice via in vitro fertilization; the resulting embryos will be transferred to foster mothers and all pups will be returned to the investigator. Purchase the females to be used as oocyte donors (the number needed will vary both by the total number of mice and the efficiency with which the strain recovers) and all associated per diem costs are the responsibility of the requesting investigator. Note: If frozen material is supplied by an outside source, there will be a $100 surcharge. Furthermore, unless the source is pre-approved, there is no guarantee of recovery of live animals.
Recovery of live mice from cryopreserved embryos An aliquot of embryos will be thawed and transferred to foster mothers; all pups will be returned to the investigator after weaning. At least one breeder pair will be provided. The investigator will be responsible for all associated per diems. Note: If frozen material is supplied by an outside source, there will be a $100 surcharge. Furthermore, unless the source is pre-approved, there is no guarantee of recovery of live animals.
Shipment of frozen material At the request of an investigator, an aliquot of his frozen material will be shipped anywhere in the world via FedEx. This can vastly simplify providing mice to collaborators. First, frozen material is unaffected by environmental conditions in transit. Secondly, in most cases frozen material is not subject to quarantine provisions, since recovery of live mice from frozen material (if properly done) serves to also re-derive the strain. The frozen material is shipped in a special container called a "liquid nitrogen dry shipper" that is capable of maintaining cryogenic temperatures for two weeks. It weighs around 25 pounds when charged, and you will be required to provide a FedEx account number to pay for shipping the samples to your recipient plus return of the shipper. Note: Thomas Jefferson University and the Office of Technology Transfer requires that you complete an MTA with the recipient before we can ship any material.
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